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Lsbad, CA). To assemble His-Ex4a(+)total expression vector, WT1 cDNALsbad, CA). To construct His-Ex4a(+)full expression vector, WT1 cDNA fragments from exons 1 to 4a and exons 5 to 10 were PCR-amplified and inserted into EcoRI/NotI and NotI/XbaI web page, respectively, of pcDNA3.1His vector. 17AA(+)KTS(-)WT1 isoform (WTB) vector and N-terminal His-tagged 17AA(+)KTS (+)WT1 isoform (His-WTD) v
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